Isolation and structure of a new factor of the vitamin B12 group: guanosine diphosphate factor B

R. Barchielli; G. Boretti; A. Di Marco; P. Julita; A. Migliacci; A. Minghetti; C. Spalla

doi:10.1042/bj0740382

February 1, 1960Biochemical JournalOpen Access

Isolation and structure of a new factor of the vitamin B12 group: guanosine diphosphate factor B

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Key Points

Characterize and isolate an unidentified violet pigment generated during the chemical synthesis and analysis of guanosine diphosphate factor B.
Attempted purification and separation of the pigment using chromatography and solvent extraction techniques.
Assessed spectroscopic and chemical reactivity by treating the reaction mixture with mineral acid across a pH range of 9.5 to 10.1.
Chromatography and solvent extraction methods were unsuccessful in isolating the violet pigment from the mixture.
Treatment with mineral acid induced a fully reversible color shift from violet to orange between pH 9.5 and 10.1.
The observed pH-dependent reversible transformation indicates an anionic pigment structure, likely governed by a keto-enol tautomeric system.

Implication

Biochemical analysis uncovers reversible pH-dependent color changes in a vitamin B12-related reaction mixture, suggesting the formation of an anionic keto-enol pigment.

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Cite This Study

Barchielli et al. (1960) studied this question.

synapsesocial.com/papers/6a717a12ce524a4339c4ff20 https://doi.org/10.1042/bj0740382

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Isolation and structure of a new factor of the vitamin B12 group: guanosine diphosphate factor B

Key Points

Implication

Discussion

Cite This Study

Isolation and structure of a new factor of the vitamin B12 group: guanosine diphosphate factor B

Key Points

Implication

Discussion

Cite This Study

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