Peripheral blood leucocytes of channel catfish Ictalurus punctatus were characterized by Wright's stain, differential cytochemical stains, and immunofluorescence; lymphocytes, thrombocytes, neutrophils, and monocytes were identified. The morphologically similar neutrophils and monocytes were distinguished with a Sudan black B stain for neutrophils and an assay of nonspecific esterase activity for monocytes. Monocytes also phagocytized polystyrene beads. Leucocytes were physically separated by cytofluorography based upon their forward and 90° light-scattering properties. One population of sorted cells contained almost exclusively lymphocytes, a second contained predominantly thrombocytes, and the third contained both neutrophils and monocytes. Multiparameter cytofluorography with mouse monoclonal antibodies to channel catfish immunoglobulin revealed that a vast majority of leucocytes exhibiting surface immunofluorescence were lymphocytes, though only about 40% of the lymphocytes contained demonstr...
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Ellsaesser et al. (1985) studied this question.