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Polymorphonuclear leukocytes were obtained from rabbit peritoneal exudates. Granule preparations from these cells were subjected to zonal fractionation through a discontinuous sucrose gradient. Azurophilic and specific granules were enzymatically characterized by peroxidase and alkaline phosphatase, respectively. Collagenase was consistently associated with the alkaline phosphatase‐containing specific granules.
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Robertson et al. (1972) studied this question.
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