What question did this study set out to answer?

To determine the detailed binding sites and precise intermolecular distances within complexes of vancomycin and ristocetin A bound to their target substrates.

March 1, 1983Journal of the American Chemical Society

Detailed binding sites of the antibiotics vancomycin and ristocetin A: determination of intermolecular distances in antibiotic/substrate complexes by use of the time-dependent NOE

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Authors

DWDudley H. WilliamsKarlsruhe Institute of Technology MWMichael P. WilliamsonUniversity of Sheffield DBDavid W. ButcherUnilever (United Kingdom)

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Implication

Biophysical analysis uncovers intermolecular distances in antibiotic-substrate complexes, highlighting the atomic architecture of vancomycin and ristocetin A binding sites.

Key Points

To determine the detailed binding sites and precise intermolecular distances within complexes of vancomycin and ristocetin A bound to their target substrates.
Measured intermolecular proton-proton proximities in antibiotic-substrate complexes using time-dependent nuclear Overhauser effect (NOE) spectroscopy.
Calculated spatial coordinates and relative orientations of ligand nuclei within the glycopeptide binding pockets.
Quantified specific intermolecular distances that define how bacterial cell wall analogues sit inside the cleft of vancomycin and ristocetin A.
Demonstrated that time-dependent NOE measurements accurately resolve conformational geometry and noncovalent interactions in glycopeptide antibiotic complexes.

Cite This Study

Williams et al. (1983) studied this question.

synapsesocial.com/papers/6a7187b535aa2c282ce2ca56 https://doi.org/10.1021/ja00343a043

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Implication

Biophysical analysis uncovers intermolecular distances in antibiotic-substrate complexes, highlighting the atomic architecture of vancomycin and ristocetin A binding sites.

Detailed binding sites of the antibiotics vancomycin and ristocetin A: determination of intermolecular distances in antibiotic/substrate complexes by use of the time-dependent NOE

Discussion

Member takes

Implication

Key Points

Cite This Study

Detailed binding sites of the antibiotics vancomycin and ristocetin A: determination of intermolecular distances in antibiotic/substrate complexes by use of the time-dependent NOE

Discussion

Member takes

Implication

Key Points

Cite This Study

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