What question did this study set out to answer?

To determine how c-Jun N-terminal kinase interacts with the forkhead transcription factor DAF-16 to control longevity and stress resistance in Caenorhabditis elegans.

March 14, 2005Proceedings of the National Academy of Sciences

JNK regulates lifespan in Caenorhabditis elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16

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Authors

SOSeung Wook OhMarine Biodiversity Institute of Korea AMArnab MukhopadhyayNational Institute of Immunology NSNenad SvrzikapaUniversity of Oxford

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Implication

Laboratory study demonstrates that JNK-1 directly phosphorylates and activates DAF-16 in roundworms, indicating a parallel pathway to insulin signaling in regulating lifespan and stress response.

Key Points

To determine how c-Jun N-terminal kinase interacts with the forkhead transcription factor DAF-16 to control longevity and stress resistance in Caenorhabditis elegans.
Conducted genetic epistasis analyses comparing JNK signaling and insulin-like signaling pathways in Caenorhabditis elegans.
Evaluated direct protein-protein binding and phosphorylation of DAF-16 by JNK-1 in vitro and in vivo.
Tracked the subcellular localization and nuclear translocation of DAF-16 following heat-stress exposure.
JNK acts as a positive regulator of DAF-16, operating in parallel with the insulin-like signaling pathway to coordinate lifespan and stress defenses.
JNK-1 physically binds to and phosphorylates DAF-16, directly facilitating its nuclear translocation under heat stress conditions.

Cite This Study

Oh et al. (2005) studied this question.

synapsesocial.com/papers/6a7196078031ec7bb1dd0857 https://doi.org/10.1073/pnas.0500749102

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Implication

Laboratory study demonstrates that JNK-1 directly phosphorylates and activates DAF-16 in roundworms, indicating a parallel pathway to insulin signaling in regulating lifespan and stress response.

JNK regulates lifespan in Caenorhabditis elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16

Discussion

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Implication

Key Points

Cite This Study

JNK regulates lifespan in Caenorhabditis elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16

Discussion

Member takes

Implication

Key Points

Cite This Study

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