What question did this study set out to answer?

November 15, 1998Journal of NeuroscienceOpen Access

G-Protein β-Subunit Specificity in the Fast Membrane-Delimited Inhibition of Ca2+Channels

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Population

Cultured rat superior cervical ganglion neurons and yeast two-hybrid system

Comparison

Intranuclear injection with DNA encoding five… vs Comparison among the different G-protein beta…

Design

Preclinical

Authors

DGDavid E. Garcı́aUniversidad Nacional Autónoma de México BLBin LiChinese Academy of Sciences RGRafael García-FerreiroMax Planck Institute of Experimental Medicine

Discussion

Member takes

Implication

Hypothesis-generating in neuronal models; leaves open any role for Gbeta selectivity in human cardiovascular calcium channel regulation.

Key Points

To identify which G-protein beta-subunit subtypes mediate voltage-dependent inhibition of N-type calcium channels and determine their channel interaction site.
Recorded whole-cell calcium currents in cultured rat superior cervical ganglion neurons after intranuclear injection of DNA encoding five distinct G-protein beta subunits (Gbeta1 through Gbeta5).
Evaluated direct protein-protein interactions using a yeast two-hybrid assay between GAL4-activation domain-fused Gbeta subunits and the intracellular I-II loop of the calcium channel alpha1 subunit.
Gbeta1 and Gbeta2 strongly produced fast voltage-dependent inhibition of calcium currents, whereas Gbeta5 produced weak inhibition, and Gbeta3 and Gbeta4 were nearly inactive.
Yeast two-hybrid analysis revealed strong direct binding of Gbeta1 and Gbeta2 to the alpha1 subunit I-II loop, weak interaction with Gbeta5, and no detectable interaction with Gbeta3 or Gbeta4.

Structured PICO

Population

Cultured rat superior cervical ganglion neurons and yeast two-hybrid system

Intervention

Intranuclear injection with DNA encoding five different G-protein beta subunits (Gbeta1, Gbeta2, Gbeta3, Gbeta4, Gbeta5)

Comparator

Comparison among the different G-protein beta subunits

Outcome

Voltage-dependent inhibition of calcium channels and protein-protein interactionssurrogate

Gbeta1 and Gbeta2 subunits are primarily responsible for the voltage-dependent inhibition of N-type calcium channels via interaction with the linker between domains I and II of the alpha1 subunit.

Cite This Study

Garcı́a et al. (1998) studied this question.

synapsesocial.com/papers/6a719d07a528af2d65c42530 https://doi.org/10.1523/jneurosci.18-22-09163.1998

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G-Protein β-Subunit Specificity in the Fast Membrane-Delimited Inhibition of Ca2+Channels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

G-Protein β-Subunit Specificity in the Fast Membrane-Delimited Inhibition of Ca2+Channels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider