A 20-minute collagenase enzyme method successfully isolates adult guinea pig ventricular cells that retain their active membrane properties for electrophysiological studies.
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Offers rapid enzymatic isolation of functional guinea pig myocytes; leaves open translation to human cells for electrophysiological research.
Single ventricular cells have been isolated from adult guinea pig heart using a collagenase enzyme method. The procedure is straightforward, takes about 20 min, and yields 40-65% rod-shaped cells which are quiescent in millimolar Ca2+ solution. Electrophysiological studies involving external ion changes and ionic channel inhibitors suggest that the cells retain their active membrane properties following this enzymatic dissociation.
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Bustamante et al. (1981) studied this question.
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