July 1, 1999AJP Heart and Circulatory Physiology

Separation of cardiomyocytes and coronary endothelial cells for cell-specific RT-PCR

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Population

Guinea pig hearts (cells isolated by collagenase digestion)

Design

Preclinical

Authors

RPRegina Preisig‐MüllerPhilipps University of Marburg MSMichael Mederos y SchnitzlerInstitute of Pharmacology CDChristian DerstParacelsus Medizinische Privatuniversität

Discussion

Member takes

Implication

May enable cell-specific analyses in guinea pig hearts; leaves open translation to human cardiac research.

Structured PICO

Population

Guinea pig hearts (cells isolated by collagenase digestion)

Intervention

Cell separation method using a custom 'cell picker' (polyethylene pipette with micromanipulator and electric miniature valve) for cell-specific RT-PCR

Outcome

Identification of specific markers (Troponin T and endothelin-1) via RT-PCR to validate cell separationsurrogate

A simple micromanipulator-based 'cell picker' method successfully separates cardiomyocytes and coronary endothelial cells from guinea pig hearts for cell-specific gene expression analysis.

Cite This Study

Preisig‐Müller et al. (1999) studied this question.

synapsesocial.com/papers/6a89464f75060194fdc978bc https://doi.org/10.1152/ajpheart.1999.277.1.h413

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Separation of cardiomyocytes and coronary endothelial cells for cell-specific RT-PCR

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Separation of cardiomyocytes and coronary endothelial cells for cell-specific RT-PCR

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider