NMR solution spatial structure of ‘short’ scorpion insectotoxin I₅A

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Two‐dimensional 500 MHz NMR study reveals the three‐dimensional structure of the insectotoxin I 5 A of Buthus eupeus in aqueous solution. The most likely set of disulfide linkages is proposed. Comparison with the single crystal structure of the ‘long’ toxin v‐3 of Centruroides sculpturatus shows similarity in their α‐helical and antiparallel β‐structure fragments.