De novo glycan sequencing by electronic excitation dissociation MS ² -guided MS ³ analysis on an Omnitrap-Orbitrap hybrid instrument

Juan Wei; Dimitris Papanastasiou; Mariangela Kosmopoulou; Athanasios Smyrnakis; Pengyu Hong; Nafisa Tursumamat; Joshua Klein; Chaoshuang Xia; Yang Tang; Joseph Zaia; Catherine E. Costello; Cheng Lin

doi:10.1039/d3sc00870c

January 1, 2023Chemical ScienceOpen Access

De novo glycan sequencing by electronic excitation dissociation MS ² -guided MS ³ analysis on an Omnitrap-Orbitrap hybrid instrument

View Full Paper

Ask AI

Bookmark

Share

Key Points

To establish a de novo glycan sequencing workflow using electronic excitation dissociation MS2 spectra to guide subsequent MS3 structural analysis on an Omnitrap-Orbitrap hybrid instrument.
Generated candidate glycan topologies and selected target precursor fragments using electronic excitation dissociation (EED) MS2 spectra.
Performed collision-induced dissociation combined with electronic excitation dissociation (CID-EED) MS3 analysis on an Omnitrap-Orbitrap hybrid mass spectrometer.
EED MS2 fragmentation successfully produced candidate topologies suitable for directing subsequent fragment isolation.
CID-EED MS3 analysis resolved and differentiated glycan isotopologies that were indistinguishable at the MS2 stage.

Implication

Experimental analysis demonstrates de novo glycan sequencing using multistage EED fragmentation, indicating reliable differentiation of structural isotopologies.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Wei et al. (2023) studied this question.

synapsesocial.com/papers/6a71b06ee36a167817e36d73 https://doi.org/10.1039/d3sc00870c

View Full Paper

Ask AI

Bookmark

Share

De novo glycan sequencing by electronic excitation dissociation MS ² -guided MS ³ analysis on an Omnitrap-Orbitrap hybrid instrument

Key Points

Implication

Discussion

Cite This Study

De novo glycan sequencing by electronic excitation dissociation MS ² -guided MS ³ analysis on an Omnitrap-Orbitrap hybrid instrument

Key Points

Implication

Discussion

Cite This Study

Keep reading

Keep reading