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A high performance liquid chromatographic (HPLC) assay is presented for the determination of albuterol in human plasma. Sample analysis is performed with a silica gel column and an aqueous mobile phase. Plasma samples are extracted with chloroform containing di(2-ethylhexyl) phosphate. Samples are back extracted into a hydrochloric acid solution. A fluorescence detector is used for this assay. the detection limit of the assay is 0.2 ng/ml for 0.5 ml plasma samples. This method has been used in a bioavailability study of albuterol sulfate tablets (4.8 mg) given to healthy volunteers.
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Wu et al. (1991) studied this question.
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