What question did this study set out to answer?

To investigate whether hypoxic macrophages produce growth factors that can stimulate the proliferation of endothelial cells under low-oxygen conditions.

May 9, 1995Proceedings of the National Academy of SciencesOpen Access

Hypoxia-mediated induction of acidic/basic fibroblast growth factor and platelet-derived growth factor in mononuclear phagocytes stimulates growth of hypoxic endothelial cells.

View Full Paper

Ask AI

Bookmark

To investigate whether hypoxic macrophages produce growth factors that can stimulate the proliferation of endothelial cells under low-oxygen conditions.
Exposed mononuclear phagocytes (macrophages) to low oxygen tension (PO2 ~12–14 torr) and analyzed conditioned media using heparin-affinity chromatography with ascending NaCl gradients.
Performed antibody neutralization assays against PDGF, aFGF, and bFGF on mitogenic fractions.
Evaluated metabolic labeling, immunoprecipitation of cell lysates/supernatants, and growth factor induction using nickel, cobalt, and sodium azide under normoxic conditions.
Hypoxic macrophage-conditioned medium resolved into three distinct mitogenic peaks specifically neutralized by antibodies against PDGF, aFGF, and bFGF, respectively.
Metabolic labeling confirmed increased synthesis and secretion of immunoprecipitable PDGF, aFGF, and bFGF in hypoxic macrophages without compromising cell viability.
Exposure of normoxic macrophages to nickel or cobalt mimicked hypoxia by inducing bFGF and PDGF release, whereas sodium azide had no effect, pointing to a heme-containing oxygen sensor.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

In vitro study reveals hypoxic macrophages secrete PDGF and FGFs to stimulate endothelial growth, indicating a key paracrine angiogenic mechanism.

Cite This Study

Kuwabara et al. (1995) studied this question.

synapsesocial.com/papers/6a71cfd126a7f98052ddfcb4 https://doi.org/10.1073/pnas.92.10.4606

View Full Paper

Ask AI

Bookmark

Implication

In vitro study reveals hypoxic macrophages secrete PDGF and FGFs to stimulate endothelial growth, indicating a key paracrine angiogenic mechanism.

Hypoxia-mediated induction of acidic/basic fibroblast growth factor and platelet-derived growth factor in mononuclear phagocytes stimulates growth of hypoxic endothelial cells.

Key Points

Discussion

Implication

Cite This Study

Hypoxia-mediated induction of acidic/basic fibroblast growth factor and platelet-derived growth factor in mononuclear phagocytes stimulates growth of hypoxic endothelial cells.

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider