Toxoplasma gondii was described originally as dividing par bipartition (Nicolle and Manceaux, 1909). In a careful study of the cytology of T. gondii, Cross (1947), using iron hematoxylin, Giemsa and Feulgen staining techniques, depicted forms of the organism which she interpreted as being part of a cycle of binary fission. Chernin and Weller (1957) observing, as other workers had done, the regularity with which parasites were found in multiples of 2 within infected tissue culture cells, also considered division of T. gondii to be by binary fission.
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Goldman et al. (1958) studied this question.