Key result
High-resolution melting analysis rapidly and efficiently detected human Torque teno viruses, revealing prevalence rates of 71.0% for TTV, 31.0% for TTMDV, and 54.0% for TTMV in healthy subjects.
Cross-Sectional (n=113)
No
High-resolution melting analysis is a rapid and efficient method for detecting and distinguishing human Torque teno viruses.
High-resolution melting analysis may aid TTV surveillance in healthy cohorts; leaves open clinical diagnostic utility.
Torque teno viruses (TTVs) are recently discovered DNA viruses, with heterogeneous genomes, highly prevalent in populations worldwide. The species that infect humans are Torque teno virus (TTV), Torque teno midi virus (TTMDV) and Torque teno mini virus (TTMV). High-resolution melting analysis (HRMA) is a sensitive and effective method for genotyping and mutation scanning. Up to now, HRMA has not been utilized for detection of TTVs. The aim of this study was to asses if HRMA is suitable for detecting TTVs variants. DNA was extracted from the blood and saliva of 13 healthy subjects for method optimization. Additionally, saliva samples from 100 healthy individuals were collected for estimating the TTVs' prevalence. Viral DNA was amplified by heminested polymerase chain reaction (PCR). Second round amplicons were used for the HRMA. The samples were analyzed using two fluorescent dyes, SYBR (®) Green I and EvaGreen®. The prevalence values for TTV, TTMDV and TTMV were 71.0, 31.0 and 54.0%, respectively. The three major melting curve patterns corresponding to TTV, TTMDV and TTMV on HRMA can be easily distinguished regardless of kit used. Our results showed that HRMA is a rapid and efficient method of detecting human TTVs.
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Spandole-Dinu et al. (2013) conducted a cross-sectional in Healthy subjects (Torque teno virus detection) (n=113). High-resolution melting analysis (HRMA) was evaluated on Prevalence of TTV, TTMDV, and TTMV. High-resolution melting analysis rapidly and efficiently detected human Torque teno viruses, revealing prevalence rates of 71.0% for TTV, 31.0% for TTMDV, and 54.0% for TTMV in healthy subjects.
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