What question did this study set out to answer?

To determine the precise intracellular localization and physical association of AMPK alpha and beta subunits with glycogen particles in intact liver tissue.

July 6, 2009Journal of Histochemistry & CytochemistryOpen Access

Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immunoelectron Microscopy

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Authors

MBMoı̈se BendayanON Semiconductor (United States)ILI g LondonUniversité de Montréal BKBruce E. KempDartmouth College

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Implication

Immunoelectron microscopy study demonstrates association of AMPK subunits with glycogen particles in rat liver tissue, suggesting direct targeting of metabolic regulators to fuel stores.

Key Points

To determine the precise intracellular localization and physical association of AMPK alpha and beta subunits with glycogen particles in intact liver tissue.
Applied immunogold cytochemistry and morphometric analysis to liver tissue from ad libitum-fed normal rats.
Optimized glycogen-retention processing using glutaraldehyde and paraformaldehyde fixation, osmium tetroxide/lead citrate postfixation, and Epon embedding, comparing this to glycogen-extracting Lowicryl preparation.
Labeling for AMPK alpha(1) and alpha(2) subunits was approximately 2-fold higher over glycogen particles than over the cytosol.
Labeling for the beta(1) subunit was 8-fold higher over glycogen particles compared with cytosol, localized specifically to the periphery of glycogen rosettes.
Extraction of glycogen during non-retentive tissue processing eliminated detectable labeling, confirming that retention of the kinase subunits depends directly on preserved glycogen architecture.

Cite This Study

Bendayan et al. (2009) studied this question.

synapsesocial.com/papers/6a71df5dc1a24a6142dbc371 https://doi.org/10.1369/jhc.2009.954016

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Implication

Immunoelectron microscopy study demonstrates association of AMPK subunits with glycogen particles in rat liver tissue, suggesting direct targeting of metabolic regulators to fuel stores.

Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immunoelectron Microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immunoelectron Microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

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