Mutational Analysis Demonstrates That ClC-4 and ClC-5 Directly Mediate Plasma Membrane Currents
Population
Xenopus oocytes and HEK293 cells
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
No immediate clinical implications; extends molecular characterization of ClC-4/5 but leaves open disease relevance.
Structured PICO
P
Population
Xenopus oocytes and HEK293 cells
I
Intervention
Expression of wild-type and mutant ClC-4 and ClC-5 channels (including point mutations N565K, S168T, E211A, E224A)
O
Outcome
Plasma membrane anion currents (voltage dependence, ion selectivity, and kinetics of activation)surrogate
ClC-4 and ClC-5 directly mediate plasma membrane chloride currents, as demonstrated by mutational analysis altering channel characteristics.
Cite This Study
Friedrich et al. (1999) studied this question.