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November 1, 1976Journal of Neurochemistry

Inhibition by Neomycin of Polyphosphoinositide Turnover in Subcellular Fractions of Guinea‐pig Cerebral Cortex in Vitro

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Authors

JSJochen SchachtUniversity of Michigan

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Overview

In vitro study reveals neomycin inhibits polyphosphoinositide turnover in guinea pig cerebral cortex fractions, suggesting ionic drug-lipid binding impairs membrane lipid metabolism.

Key Points

  • To determine how neomycin and structurally related cationic compounds influence polyphosphoinositide metabolism and turnover in subcellular fractions of guinea pig cerebral cortex.
  • Incubated guinea pig cerebral cortex subcellular fractions with neomycin at concentrations from 10^-5 M to 10^-3 M in the presence of [gamma-32P]ATP.
  • Tested related aminoglycosides, neamine, spermine, poly-L-lysine, local anesthetics, and small polyamines while measuring lipid hydrolysis, suspension turbidity, and pH changes.
  • Neomycin (10^-5 M to 10^-3 M) increased phosphatidylinositol phosphate labeling and decreased phosphatidylinositol diphosphate labeling across all subcellular fractions via its cationic form.
  • Neomycin, neamine, related aminoglycosides, spermine, and poly-L-lysine inhibited the enzymatic hydrolysis of 32P-polyphosphoinositides, increased lipid suspension turbidity, and decreased suspension pH.

Cite This Study

Jochen Schacht (1976) studied this question.

synapsesocial.com/papers/6a71e8f8febe604dd70a582ehttps://doi.org/10.1111/j.1471-4159.1976.tb00318.x
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