What question did this study set out to answer?

To characterize the M1 and M2 activation states of human adipose tissue macrophages in obesity across subcutaneous and omental depots, and to assess phenotypic changes following bariatric surgery.

October 17, 2009The Journal of Clinical Endocrinology & MetabolismOpen Access

Human Adipose Tissue Macrophages: M1 and M2 Cell Surface Markers in Subcutaneous and Omental Depots and after Weight Loss

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Why the study?

Does gastric surgery alter the activation state of adipose tissue macrophages in obese women?

Population

16 premenopausal obese women and 10 nonobese women

Comparison

Gastric surgery vs Baseline (pre-surgery) and nonobese controls

Design

Cohort

Follow-up

3 months

Authors

JAJudith Aron‐WisnewskyGeneral / Preventive / Lipids JTJoan TordjmanIcahn School of Medicine at Mount Sinai CPChristine PoitouDélégation Paris 5

Discussion

Member takes

Implication

May reduce adipose inflammation after gastric bypass in obesity; hypothesis-generating and should not yet change practice.

Key Points

To characterize the M1 and M2 activation states of human adipose tissue macrophages in obesity across subcutaneous and omental depots, and to assess phenotypic changes following bariatric surgery.
Conducted a prospective single-center study in 16 premenopausal obese women (mean BMI 43.8 ± 3.4 kg/m², aged 41.1 ± 8.6 years) undergoing gastric surgery, with follow-up subcutaneous biopsies collected at 3 months.
Obtained baseline subcutaneous biopsies from 10 nonobese female controls (mean BMI 22.5 ± 0.75 kg/m², aged 43.3 ± 3.5 years).
Quantified macrophage surface expression of CD40 (M1 proinflammatory marker) and CD206 and CD163 (M2 anti-inflammatory markers) using immunohistochemistry.
CD40(+) macrophage infiltration was significantly elevated in obese compared with nonobese subjects, and was significantly higher in omental versus subcutaneous adipose tissue in obese women.
CD163(+) and CD206(+) macrophage numbers showed no significant differences based on obesity status or anatomical fat pad location.
At 3 months post-surgery, the ratio of CD40(+) to CD206(+) macrophages decreased 2-fold in subcutaneous fat compared to pre-surgery levels (P < 0.001), reflecting a concurrent decrease in CD40(+) cells and increase in CD206(+) cells.

Structured PICO

Does gastric surgery alter the activation state of adipose tissue macrophages in obese women?

Population

16 premenopausal obese women (aged 41.1 +/- 8.6 yr; BMI 43.8 +/- 3.4 kg/m2) and 10 nonobese women (aged 43.3 +/- 3.5 yr; BMI 22.5 +/- 0.75 kg/m2)

Intervention

Gastric surgery

Comparator

Baseline (pre-surgery) and nonobese controls

Outcome

Number of macrophages stained with CD40, CD206, and CD163 surface markerssurrogate

Weight loss via gastric surgery shifts adipose tissue macrophages from a proinflammatory M1 state to a less proinflammatory M2 state.

Cite This Study

Aron‐Wisnewsky et al. (2009) studied this question.

synapsesocial.com/papers/6a71f0df78a11c550e0bdf16 https://doi.org/10.1210/jc.2009-0925

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Human Adipose Tissue Macrophages: M1 and M2 Cell Surface Markers in Subcutaneous and Omental Depots and after Weight Loss

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Human Adipose Tissue Macrophages: M1 and M2 Cell Surface Markers in Subcutaneous and Omental Depots and after Weight Loss

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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