What question did this study set out to answer?

To model human papillomavirus-induced neoplastic progression by targeting HPV16 early region gene expression to basal squamous epithelial cells in transgenic mice.

July 1, 1994Journal of VirologyOpen Access

Progressive squamous epithelial neoplasia in K14-human papillomavirus type 16 transgenic mice

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Authors

JAJ M ArbeitWashington University in St. Louis KMKarl MüngerTufts University PHPeter M. HowleyRutgers, The State University of New Jersey

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Implication

Transgenic animal study demonstrates progressive squamous neoplasia in HPV16-expressing mice, indicating a reliable model for HPV-induced carcinogenesis.

Key Points

To model human papillomavirus-induced neoplastic progression by targeting HPV16 early region gene expression to basal squamous epithelial cells in transgenic mice.
Targeted expression of wild-type or E1/E2-mutant HPV16 early regions to basal squamous epithelial cells using a human keratin 14 (K14) promoter, generating 21 founder mice and establishing eight transgenic lines.
Evaluated histological progression across skin and mucosal sites using 5-bromo-2'-deoxyuridine (BrdU) incorporation to quantify DNA synthesis and immunohistochemistry for K5, K14, K10, and filaggrin to track differentiation.
Mice developed progressive lesions starting from mild hyperplasia to severe hyperplasia, dysplasia, and papillomatosis across multiple sites, with the ears showing 100% penetrance across all transgenic lines.
The absence of E1 or E2 gene expression did not alter the severity of the phenotype, as intact E6 and E7 oncogenes sustained neoplastic transformation.
Histopathological progression corresponded with incremental increases in cellular DNA synthesis and severe disruption of terminal keratinocyte differentiation.

Cite This Study

Arbeit et al. (1994) studied this question.

synapsesocial.com/papers/6a7204f36ceb2bbd16e0472a https://doi.org/10.1128/jvi.68.7.4358-4368.1994

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Implication

Transgenic animal study demonstrates progressive squamous neoplasia in HPV16-expressing mice, indicating a reliable model for HPV-induced carcinogenesis.

Progressive squamous epithelial neoplasia in K14-human papillomavirus type 16 transgenic mice

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Cite This Study

Progressive squamous epithelial neoplasia in K14-human papillomavirus type 16 transgenic mice

Discussion

Member takes

Implication

Key Points

Cite This Study

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