June 1, 1993Journal of Biological ChemistryOpen Access

Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum

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Population

Sarcoplasmic reticulum (SR) vesicles isolated from AT-1 cardiomyocytes and mouse ventricular SR vesicles

Design

Preclinical

Authors

LJLarry R. JonesUniversity of Vermont LFLoren J. FieldUniversity of North Carolina at Chapel Hill

Discussion

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Implication

Cytoplasmic PLB domain insufficient for SR Ca2+ pump inhibition; leaves open transmembrane domain requirement in human models.

Structured PICO

Population

Sarcoplasmic reticulum (SR) vesicles isolated from AT-1 cardiomyocytes and mouse ventricular SR vesicles

Intervention

Phospholamban (PLB) synthetic peptide consisting of amino acid residues 2-25 (up to 0.44 mM) and monoclonal antibody recognizing PLB residues 9-17

Outcome

Ca2+ transport/uptake by SR vesiclessurrogate

The cytoplasmic domain of phospholamban containing phosphorylation sites is insufficient to inhibit the cardiac SR Ca2+ pump, indicating that the transmembrane region is also essential for Ca2+ transport regulation.

Cite This Study

Jones et al. (1993) studied this question.

synapsesocial.com/papers/6a720ca75d37378ac1defc0e https://doi.org/10.1016/s0021-9258(19)50222-8

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Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider