What question did this study set out to answer?

To determine the mechanism by which glucocorticoids regulate mitogen-stimulated interleukin 3 expression in cloned murine helper T lymphocytes.

November 1, 1985The Journal of ImmunologyOpen Access

Regulation of IL 3 expression by glucocorticoids in cloned murine T lymphocytes.

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Authors

JCJanice CulpepperGates Foundation FLF LeeInstitut de Biologie Moléculaire et Cellulaire

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Implication

In vitro study reveals dexamethasone suppresses mitogen-induced interleukin-3 gene transcription in murine helper T cells, indicating direct glucocorticoid control over cytokine expression.

Key Points

To determine the mechanism by which glucocorticoids regulate mitogen-stimulated interleukin 3 expression in cloned murine helper T lymphocytes.
Stimulated the cloned murine helper T cell line Cl.Ly-1+2-/9 with concanavalin A in the presence or absence of dexamethasone.
Assessed interleukin 3 bioactivity in culture supernatants and evaluated the kinetics, stability, processing, and nuclear transport of induced cytokine mRNA.
Dexamethasone rapidly blocked concanavalin A-induced interleukin 3 biological activity and cellular mRNA accumulation, as well as granulocyte-macrophage colony-stimulating factor mRNA, without affecting constitutive Thy-1 mRNA synthesis.
Interleukin 3 mRNA degradation rates, processing, and nucleocytoplasmic transport were unaffected by hormone exposure, indicating direct transcriptional repression of the interleukin 3 gene.

Cite This Study

Culpepper et al. (1985) studied this question.

synapsesocial.com/papers/6a720e99f44fa9f079dfaa62 https://doi.org/10.4049/jimmunol.135.5.3191

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Implication

In vitro study reveals dexamethasone suppresses mitogen-induced interleukin-3 gene transcription in murine helper T cells, indicating direct glucocorticoid control over cytokine expression.

Regulation of IL 3 expression by glucocorticoids in cloned murine T lymphocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

Regulation of IL 3 expression by glucocorticoids in cloned murine T lymphocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

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