What question did this study set out to answer?

The study aims to evaluate the effects of diabetes on endothelial function in renal arteries using a rat model.

December 1, 1993Journal of the American Society of Nephrology

Diabetes-induced endothelial dysfunction in streptozotocin-treated rats

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Why the study?

Does streptozotocin-induced diabetes impair endothelium-dependent relaxations in the renal arteries of rats?

Population

Wistar-Kyoto rats

Comparison

Diabetes induced by intravenous injection of… vs Control (non-diabetic) rats

Design

Preclinical

Follow-up

6 and 16 weeks

Authors

FDF X DaiUniversity of Kansas Medical Center ADA. DiederichJohns Hopkins University JSJ. SkopecUniversity of Kansas Medical Center

Discussion

Member takes

Implication

Impaired endothelial relaxation in diabetic rat renal arteries should not change practice; hypothesis-generating for oxidative mechanisms in diabetic nephropathy.

Key Points

The study aims to evaluate the effects of diabetes on endothelial function in renal arteries using a rat model.
Streptozotocin (55 mg/kg) was administered intravenously to induce diabetes in Wistar-Kyoto rats.
Vasoactive responses of renal arteries from diabetic and control rats were compared using arteriograph assemblies.
Relaxations in response to stimuli (acetylcholine and histamine) were measured after 6 and 16 weeks of diabetes.
Diabetic rats required significantly higher concentrations of acetylcholine for 50% relaxation than control rats at both 6 weeks (3.2 versus 0.4 μM) and 16 weeks (25 versus 0.4 μM), showing 8- and 62-fold decreases in sensitivity, respectively.
After 6 weeks, renal arteries from diabetic rats were also 20-fold less sensitive to histamine.
Treatment with the hydroxyl radical scavenger dimethylthiourea normalized acetylcholine-induced relaxations in diabetic arteries.

Structured PICO

Does streptozotocin-induced diabetes impair endothelium-dependent relaxations in the renal arteries of rats?

Population

Wistar-Kyoto rats

Intervention

Diabetes induced by intravenous injection of streptozotocin (55 mg/kg)

Comparator

Control (non-diabetic) rats

Outcome

Vasoactive responses of renal arteries in vitro (endothelium-dependent relaxations mediated by nitric oxide)surrogate

Diabetes impairs endothelium-dependent relaxation in rat renal arteries due to increased production of free radicals and prostaglandin endoperoxides that oppose the vasorelaxant effects of nitric oxide.

Cite This Study

Dai et al. (1993) studied this question.

synapsesocial.com/papers/6a7210b0febe604dd70a6bd3 https://doi.org/10.1681/asn.v461327

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Implication

Impaired endothelial relaxation in diabetic rat renal arteries should not change practice; hypothesis-generating for oxidative mechanisms in diabetic nephropathy.

Diabetes-induced endothelial dysfunction in streptozotocin-treated rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Diabetes-induced endothelial dysfunction in streptozotocin-treated rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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