COMPARATIVE STUDY OF THE CHIRAL RESOLUTION OF β-BLOCKERS ON CELLULOSE TRIS (3,5-DIMETHYL-PHENYLCARBAMATE) PHASES IN NORMAL AND REVERSED PHASE MODES

The chiral separation of β-blockers on cellulose tris (3,5-dimethylphenylcarbamate) columns is described. The separation power of a Chiracel OD normal phase column, a Chiracel OD-RH reversed phase column, and a Chiracel ODRH reversed phase narrow-bore column were compared. Hexane–2-propanol–diethylamine mixtures were used as mobile phase in the normal phase mode and sodium perchlorate buffer containing different amounts of acetonitrile in the reversed phase mode. Sixteen μ-blockers were resolved under normal phase conditions and eleven under reversed phase conditions. The values of α and Rs on normal phase column ranged from 4.46 to 1.10 and 10.04 to 0.97, respectively. The values of α and Rs on reversed phase columns varied from 4.04 to 1.04 and 3.98 to 0.40(on normal bore column) and 4.36 to 1.06 and 2.26 to 0.40(on narrow bore column).