Lipolytic activity toward long-chain triglycerides was studied in the lateral line muscle of rainbow trout (Salmo gairdneri) by use of tissue slices. Emulsification of substrates with phospholipids was found to be necessary to demonstrate lipolysis, which occurred in decreasing intensity with tripalmitin, triolein, and tristearin. The lipolytic activity showed optimum activity at pH 7.3 and it was inhibited by NaF, protamine, and p-chloromercuriphenyl sulfonate. Albumin had little effect on the activity. Epinephrine did not stimulate lipolysis, but it reduced loss of activity during preincubation.
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Bilinski et al. (1969) studied this question.