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Mouse blood monocytes grow well in liquid but poorly in agar medium. In this study the effect of haemolysate on the clonal growth of blood monocytes in agar cultures was investigated. The results suggested that haemolysate from mouse or rat red blood cells contains a factor(s) that can render the murine blood monocytes able to grow well in agar culture (anchorage-independent growth). The mechanism of action of the enhancing factor(s) is discussed.
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Chen et al. (1980) studied this question.
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