Analysis of PANoptosis-Related LncRNA-miRNA-mRNA Network Reveals LncRNA SNHG7 Involved in Chemo-Resistance in Colon Adenocarcinoma

Figures

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  Figure 1. Workflow Diagram.

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  Figure 2. Identifying Metastasis- and PANoptosis-Related lncRNAs in COAD. (A) Venn diagram identifying metastasis-related genes, PANoptosis-related lncRNAs, and differentially expressed genes in TCGA-COAD. (B) Heatmap showing the differential expression of 9 candidate lncRNAs in COAD and adjacent normal tissues. (C) The correlation heatmap showing the correlation between 9 candidate lncRNAs and PANoptosis-related mRNAs. (D) Sankey diagram visualizes the degree of relationship between PANoptosis-related mRNAs, 9 candidate lncRNAs, and COAD prognosis.

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  Figure 3. Construction of ceRNA Network of Candidate lncRNAs.

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  Figure 4. Functional and Pathway Enrichment Analysis of mRNAs in the lncRNA-miRNA-mRNA Network. (A–C) The mRNAs associate with three GO categories: biological process, cellular component, and molecular function. The most significant GO terms for lncRNA-related mRNAs are regulation of mRNA metabolic process, transferase complex, transferring phosphorus-containing groups, and protein serine/threonine kinase activity. (D, E) KEGG pathway analysis reveals mRNA-enriched associated signaling pathways. The most enriched KEGG pathway for lncRNA-associated mRNAs is the cancer.

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  Figure 5. Immune Infiltration Analysis of Candidate PANoptosis- and Metastasis-Related lncRNAs. (A) Correlation of candidate lncRNAs with different tumor-infiltrating immune cells. (B) Immune cell infiltration in high and low lncRNA expression groups. (C) Comparison of ESTIMATE, immune, and stromal scores in candidate lncRNA high and low expression groups. *P < 0.05; **P < 0.01; ***P < 0.001; ns P > 0.05 (ns, not significant).

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  Figure 6. Survival, Expression, and Mutational Analysis of lncRNA SNHG7 in COAD. (A) Forest plot showing the results of multivariate Cox regression analysis of 9 lncRNAs and overall survival in COAD patients. (B) Kaplan-Meier survival analysis of SNHG7. (C) SNHG7 expression in COAD. (D, E) The relationship between SNHG7 expression in COAD with tumor stage and lymph node metastasis. (F) Copy number variation (CNV) type distribution of SNHG7 in COAD. (G) Correlation between SNHG7 CNV and expression in COAD (H) Overall survival of SNHG7 CNV in COAD.

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  Figure 7. Drug sensitivity analysis of SNHG7.