[Effect of moxibustion at "acupoint-open on-time" on immune function and nuclear factor-κB signaling pathway in aging rats].

OBJECTIVE: To observe the effect of moxibustion at "acupoint-open on-time" （Linggui Bafa） on immune function and nuclear factor-κB (NF-κB) signaling pathway in aging rats, so as to reveal its underlying mechanism of anti-agingt. METHODS: =10 in each group). Aging model was established by intraperitoneal injection of D-galactose (300 mg/kg) for 42 days. Moxibustion was applied at "acupoint-open on-time" or CV8 once daily for 28 days. The serum levels of TNF-α, IgG, IgA and IgM were detected by ELISA. The spleen index of rats was calculated. The expression of NF-κB p65 and NF-κB inhibitor protein (IκB) α mRNA of spleen tissues was detected by real-time PCR. CONCLUSION: Moxibustion at both "acupoint-open on-time" and CV8 can enhance the immune function of aging rats, which may be related to its effects in inhibiting NF-κB signaling pathway.