What question did this study set out to answer?

Investigate the genetic control of asparagine accumulation and breakdown across grain tissues in contrasting bread wheat genotypes under differing sulphur supply conditions.

August 1, 2019BMC GenomicsOpen Access

Contrasting gene expression patterns in grain of high and low asparagine wheat genotypes in response to sulphur supply

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Authors

TCTanya Y. CurtisA-T Society SRSarah RaffanRothamsted Research YWYongfang WanRothamsted Research

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Implication

Gene expression analysis reveals tissue-specific regulation of asparagine metabolism under sulphur deficiency in wheat, suggesting the embryo drives free asparagine accumulation.

Key Points

Investigate the genetic control of asparagine accumulation and breakdown across grain tissues in contrasting bread wheat genotypes under differing sulphur supply conditions.
Conducted RNA-seq transcriptomic profiling on embryo and endosperm tissues of two bread wheat genotypes, Spark and SR3.
Sampled grain tissues at 14 and 21 days post anthesis under conditions of sulphur sufficiency and deficiency.
TaASN2 showed the highest expression among asparagine synthetase genes, with substantially higher transcript abundance in the embryo than in the endosperm.
Under sulphur deficiency at 21 days post anthesis, embryo TaASN2 and glutamine synthetase expression increased while asparaginase expression decreased in SR3, whereas Spark did not display this response.
Sulphur deficiency altered the expression of protein kinase genes SnRK1 and GCN2, as well as bZIP transcription factors (including bZIP9, bZIP25, and bZIP63) containing SnRK1 target sites.

Cite This Study

Curtis et al. (2019) studied this question.

synapsesocial.com/papers/6a7234eeb27f15817827f8ae https://doi.org/10.1186/s12864-019-5991-8

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Implication

Gene expression analysis reveals tissue-specific regulation of asparagine metabolism under sulphur deficiency in wheat, suggesting the embryo drives free asparagine accumulation.

Contrasting gene expression patterns in grain of high and low asparagine wheat genotypes in response to sulphur supply

Discussion

Member takes

Implication

Key Points

Cite This Study

Contrasting gene expression patterns in grain of high and low asparagine wheat genotypes in response to sulphur supply

Discussion

Member takes

Implication

Key Points

Cite This Study

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