Why the study?
Primary adult cardiomyocyte culture is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible.
The study establishes a novel in vitro culture method for adult mouse cardiomyocytes that preserves their functional and structural properties for extended experiments.
Enables extended functional studies in adult cardiomyocytes; leaves open translation to human disease and clinical application.
Primary adult cardiomyocyte (aCM) culture is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca 2+ cycling to enable physiologically-relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations that probe mechanisms underlying myocyte functional performance.
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Callaghan et al. (2019) studied this question.
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