Why the study?
Efficient replication of African swine fever virus requires a suitable cell culture supporting isolation even at low viral loads from field samples.
The described protocol provides a highly sensitive method for isolating African swine fever virus from field samples using porcine bone marrow primary cell culture.
May facilitate ASFV isolation from low-titer samples; leaves open validation before routine adoption.
Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load, as in the case of isolating the virus from field samples. This article presents a detailed protocol on the preparation of porcine bone marrow primary (PBMP) cell culture, which has a high sensitivity towards ASFV, resulting in high viral yields with a minimal risk of bacterial contamination.
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Puzankova et al. (2023) studied this question.
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