What question did this study set out to answer?

To identify the specific surface antigen recognized by the monoclonal antibody OKT-9 on human acute lymphocytic leukemia cells.

September 1, 1981The Journal of Immunology

Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells.

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Authors

JGJames W. GodingMonash University GBG F BurnsUniversity of Newcastle Australia

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Implication

Biochemical analysis demonstrates that monoclonal antibody OKT-9 targets the transferrin receptor on human lymphocytes, suggesting its utility as a biomarker for dividing cells.

Key Points

To identify the specific surface antigen recognized by the monoclonal antibody OKT-9 on human acute lymphocytic leukemia cells.
Precipitated human lymphocyte surface proteins using either the OKT-9 monoclonal antibody or transferrin-agarose beads.
Characterized molecular weights under intact and reducing conditions and conducted peptide mapping via limited proteolysis to compare precipitated proteins.
OKT-9 recognized a surface protein with a molecular weight of 200,000 intact and 95,000 reduced, indicative of a disulfide-bonded homodimer.
Limited proteolysis peptide mapping demonstrated structural homology between proteins isolated by OKT-9 antibodies and transferrin-agarose, confirming OKT-9 binds the transferrin receptor.

Cite This Study

Goding et al. (1981) studied this question.

synapsesocial.com/papers/6a723bd05671bbf00bc30982 https://doi.org/10.4049/jimmunol.127.3.1256

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Implication

Biochemical analysis demonstrates that monoclonal antibody OKT-9 targets the transferrin receptor on human lymphocytes, suggesting its utility as a biomarker for dividing cells.

Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

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