The Role of AIF-1 in the Aldosterone-Induced Vascular Calcification Related to Chronic Kidney Disease: Evidence From Mice Model and Cell Co-Culture Model

Figures

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  Figure 1. The serum Cr, BUN, Ca2+, AIF-1, and Aldo levels in CKD mice model (A–E). The histomorphology of renal tissue(400X) (F) and thoracic aorta(400X) (G). VonKossa staining(400X) (H) and quantitative analysis (I). (n=6) (*P<0.05, Control group vs. CKD group; #P<0.05, CKD group vs. CKD+Spir group).

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  Figure 2. The serum FGF23, Phosphate, and PTH levels in the CKD mice model (A–C) and AIF-1KO mice model (D–F). (n=6) (*P<0.05, Control group vs. CKD group; #P<0.05, CKD group vs. CKD+Spir group or CKD group vs. AIF-1KO+CKD group).

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  Figure 3. Cell apoptosis in the vascular tissue using TUNEL staining (200X) (A, B). Western blot and PCR analysis (C–L). (n=6) (*P<0.05, Control group vs. CKD group; #P<0.05, CKD group vs. CKD+Spir group).

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  Figure 4. The serum Cr, BUN, Ca2+, AIF-1, and Aldo levels in the AIF-1KO mice model (A–E). The histomorphology of renal tissue(400X) (F) and thoracic aorta (400X) (G). VonKossa staining(400X) (H) and quantitative analysis (I). (n=6) (*P<0.05, Control group vs. CKD group; #P<0.05, CKD group vs. AIF-1KO+CKD group).

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  Figure 5. Cell apoptosis in the vascular tissue using TUNEL staining (200X) (A, B). Western blot and PCR analysis (C–J). (n=6) (*P<0.05, Control group vs. CKD group; #P<0.05, CKD group vs. AIF-1KO+CKD group).

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  Figure 6. Calcium content using flow cytometric (A, B). The positive area of ALP staining (200X) (C). (n=6) (*P<0.05, NC group vs. Aldo+ NC groups; #P<0.05, Aldo+NC group vs. Aldo+ shAIF-1 groups).

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  Figure 7. Cell apoptosis using TUNEL analysis (200X) (A, C). Western blot and PCR analysis (B, D–J). (n=6) (*P<0.05, NC group vs. Aldo+NC groups; #P<0.05, Aldo+NC group vs. Aldo+ shAIF-1 groups).