Quercetin Ameliorate Oxidative Stress-Induced Small Intestine Toxicity by Mefenamic Acid

The objectives of this study were to determine the involvement of oxidative stress and the ameliorative effect of quercetin (QUR) on mefenamic acid-induced small intestine toxicity by measuring the level of superoxide radical, hydrogen peroxide (H2O2), protein carbonylation (PC), and advanced oxidation protein products (AOPPs). In this experiment, a small intestine was taken from male rats (Rattus novergicus). Samples then homogenized and divided into three groups with; T1 served as control which contains small intestine homogenate only; T2 which contains small intestine homogenate + 500 mg of mefenamic acid; and T3 which contains small intestine homogenate + 500 mg of mefenamic acid + 250 mg/L of quercetin. After treatment, every 15-minute superoxide radical, H2O2, PC, and AOPPs levels were estimated. The results revealed that mefenamic acid increased the level of superoxide radical, H2O2, PC, and AOPPs, while QUR decreases the level of all parameters. These results indicated that mefenamic acid induce small intestine toxicity through oxidative stress mechanisms as can be seen from the increasing of superoxide radical, H2O2, PC, and AOPPs levels. Also, the results indicated that QUR could inhibit these process as can be seen from the decreasing of all parameters.