Immobilized Firefly Luciferase and its use in Analysis

Firefly luciferase immobilized on sepharose 4B and cellophane filmis shown to retain as much as 20% of its initial activity and can be durably stored at 4O°C with no appreciable loss in its enzymatic activity. The application of the immobilized luciferase is described for ATP detection at concentrations ranging from 0.1 pM to 1 mM, as well as for activity determination of enzymes catalyzing ATP synthesis (pyruvatekinase) and ATP hydrolysis (ATP-ase). The same sample of luciferase immobilized on cellophane film was 7%.