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April 1, 1986Journal of VirologyOpen Access

Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals

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Population

Sera from individuals positive in an enzyme-linked immunosorbent assay for antibodies to acquired immune…

Design

Preclinical

Authors

KSKathelyn S. SteimerChiron (Norway)KHKeith HigginsApple (Israel)MPMaureen A. PowersEmory University

Discussion

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Implication

p31 ELISA may detect ARV antibodies in most seropositive samples; leaves open diagnostic validation in clinical use.

Structured PICO

P
Population
Sera from individuals positive in an enzyme-linked immunosorbent assay (ELISA) for antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV)
I
Intervention
Recombinant polypeptide from the endonuclease region of the ARV polymerase (pol) gene (expressed directly and as a fusion protein with human superoxide dismutase)
O
Outcome
Detection of serum antibodies to p31 using the developed ELISAsurrogate

A recombinant p31 protein from the ARV pol gene can be used in an ELISA to detect antibodies in the majority of individuals infected with the AIDS-associated retrovirus.

Cite This Study

Steimer et al. (1986) studied this question.

synapsesocial.com/papers/6a724d073ce530166bc34ad2https://doi.org/10.1128/jvi.58.1.9-16.1986
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Also Consider

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