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January 26, 2024MetabolitesOpen Access

Associations of the Single Bovine Embryo Growth Media Metabolome with Successful Pregnancy

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Authors

ETElina TsoppKKKalle KilkETEgon Taalberg

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Overview

Prospective cohort study uncovers differential culture media metabolites in bovine blastocysts, indicating potential biomarkers for predicting successful embryo implantation.

Key Points

  • To determine whether metabolomic fingerprints of bovine embryo growth media can improve the prediction of successful embryo implantation.
  • Conducted a prospective cohort study analyzing culture media from in vitro-produced day 7 blastocysts with successful implantation (n = 11), failed implantation (n = 10), and embryo-free control media (n = 5).
  • Measured 189 metabolites per sample using an AbsoluteIDQ p180 Targeted Metabolomics Kit paired with liquid chromatography-tandem mass spectrometry (LC-MS/MS).
  • Blastocysts yielding successful implantation exhibited significantly higher methionine sulfoxide (p < 0.001), DOPA (p < 0.05), spermidine (p < 0.001), acetylcarnitine-to-free-carnitine ratio (p < 0.05), and (C2 + C3)-to-free-carnitine ratio (p < 0.05), alongside lower threonine (p < 0.001) and phosphatidylcholine PC ae C30:0 (p < 0.001) relative to control media.
  • Direct comparison between successfully implanted embryos and those that failed revealed significant differentiation restricted to DOPA, spermidine, C2/C0 ratio, (C2 + C3)/C0 ratio, and PC ae C30:0.

Cite This Study

Tsopp et al. (2024) studied this question.

synapsesocial.com/papers/6a72536ffebe604dd70a8af7https://doi.org/10.3390/metabo14020089
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Also Consider

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  5. 5In vitro production significantly reduces metabolic differences among bovine embryos2025 · 3 citations