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May 5, 2016Stem Cell ReportsOpen Access

Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

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Population

Human induced pluripotent stem cell-derived cardiomyocytes, including a patient-derived cell line carrying…

Comparison

Substrate nanopatterning approach to produce… vs Human fetal myofibrils of similar gestational…

Design

Preclinical

Authors

JPJosè Manuel PionerHeart Failure / CardiomyopathyARAlice Ward RaccaUniversity of Massachusetts LowellJKJordan M. KlaimanUniversity of Washington

Discussion

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Implication

Enables adult-like hiPSC-CM contractility studies; leaves open clinical translation pending validation.

Structured PICO

P
Population
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), including a patient-derived cell line carrying the familial cardiomyopathy MYH7 mutation (E848G)
I
Intervention
Substrate nanopatterning approach to produce hiPSC-CMs with adult-like dimensions, followed by isolation of myofibrils and mechanical measurements using a custom-built apparatus
C
Comparator
Human fetal myofibrils of similar gestational age and adult preparations
O
Outcome
Tension and kinetics of activation and relaxationsurrogate

A novel substrate nanopatterning and isolation approach enables detailed mechanical measurements of hiPSC-CM myofibrils, facilitating the study of cardiomyopathic mutations.

Cite This Study

Pioner et al. (2016) studied this question.

synapsesocial.com/papers/6a7260b2fe4101aa97e0d5b7https://doi.org/10.1016/j.stemcr.2016.04.006

Topics

Cardiomyopathy
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Also Consider

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  1. 1Patient-Specific Induced Pluripotent Stem Cells as a Model for Familial Dilated Cardiomyopathy2012 · 706 citations
  2. 2Troponin I gene expression during human cardiac development and in end-stage heart failure.1993 · 258 citations
  3. 3Contractile properties of developing human fetal cardiac muscle2015 · 64 citations
  4. 4Atomic Force Mechanobiology of Pluripotent Stem Cell-Derived Cardiomyocytes2012 · 141 citations
  5. 5Effects of Chronic Atrial Fibrillation on Active and Passive Force Generation in Human Atrial Myofibrils2010 · 57 citations