What question did this study set out to answer?

To characterize the cytokine expression profile of immortalized, nontransformed Met murine hepatocyte (MMH) cell lines and assess their capacity to support in vitro hematopoiesis.

December 1, 1998HepatologyOpen Access

Hematopoietic Support and Cytokine Expression of Murine–Stable Hepatocyte Cell Lines (Mmh)

View Full Paper

Ask AI

Bookmark

Authors

AAAlessandro AiutiVita-Salute San Raffaele University CCCarla CicchiniIstituto Pasteur SBSilvia BernardiniUniversity of Pisa

Discussion

Member takes

Implication

In vitro study demonstrates hematopoietic support and cytokine expression by murine hepatocyte lines, indicating hepatocytes directly sustain blood cell growth and differentiation.

Key Points

To characterize the cytokine expression profile of immortalized, nontransformed Met murine hepatocyte (MMH) cell lines and assess their capacity to support in vitro hematopoiesis.
Assessed cytokine gene expression in MMH lines via reverse-transcription polymerase chain reaction (RT-PCR) and ribonuclease protection assays.
Cultured progenitor-enriched fetal liver hematopoietic cells atop MMH feeder layers for up to 6 weeks to evaluate cell proliferation, colony formation, and long-term differentiation.
MMH cell lines expressed multiple cytokines critical for progenitor self-renewal and lineage differentiation, including stem cell factor, FLT3 ligand, and thrombopoietin.
Coculturing induced a 40-fold to 80-fold expansion of total hematopoietic cells and a 2.5-fold expansion of clonogenic progenitors within 1 to 2 weeks.
Hematopoiesis was maintained for up to 6 weeks with continuous precursor maturation, retaining clonogenic progenitors at 20% of initial input levels at week 5.

Cite This Study

Aiuti et al. (1998) studied this question.

synapsesocial.com/papers/6a7261205671bbf00bc30ff8 https://doi.org/10.1002/hep.510280626

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates hematopoietic support and cytokine expression by murine hepatocyte lines, indicating hepatocytes directly sustain blood cell growth and differentiation.

Hematopoietic Support and Cytokine Expression of Murine–Stable Hepatocyte Cell Lines (Mmh)

Discussion

Member takes

Implication

Key Points

Cite This Study

Hematopoietic Support and Cytokine Expression of Murine–Stable Hepatocyte Cell Lines (Mmh)

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider