Why the study?
Does cyclochlorotine affect myofibrils in cardiomyocytes and actin filament bundles in fibroblasts in vitro?
Does cyclochlorotine affect myofibrils in cardiomyocytes and actin filament bundles in fibroblasts in vitro?
Cyclochlorotine induces dose-dependently reversible disruption of myofibrils and cytoskeletal proteins in cardiomyocytes and fibroblasts in vitro.
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Cyclochlorotine may pose reversible cardiotoxicity risk in vitro; hypothesis-generating and requires in vivo validation before any clinical consideration.
The effects of cyclochlorotine (CC), a secondary metabolite of Penicillium islandicum, on cardiomyocytes and fibroblasts were investigated by fluorescent and electron microscopy. The in vitro application of CC on myocytes induced disruption of myofibrils and large accumulations of actin, myosin, alpha-actinin, and vinculin at the cellular processes. This agent also induced the formation of islands of myosin and alpha-actinin aggregates. Actin filament bundles in fibroblasts were either unaffected or disrupted completely, suggesting the presence of two, physiocochemically different, populations of fibroblasts. CC damage was dose-dependently reversible. Further studies will be required to explore the feasibility of using CC for analyzing the properties of contractile and/or cytoskeletal proteins.
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Zhou et al. (1994) studied this question.
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