Influence of melatonin introduction onthe state of glutathione system and the level of hydrogen sulfide in blood of ratswith alloxan induced diabetes mellitus

Topicality. Hyperglycemia in diabetes mellitus (DM) leads to the growth of complications associated with increasedoxidative stress. The effect of melatonin on antioxidant status in the case of diabetes mellitus is of great interest in recent years.Aim. To study the effect of melatonin administration on glutathione system parameters and the level of hydrogensulfide in blood of rats with aloxane-induced diabetes mellitus.Materials and methods. Experiments were conducted on white, non-breeding, sexually mature male rats with a body weight of 0.15-0.18 kg white male rats. Diabetes mellitus was caused by intravaginal administration of a 5 % solution of alloxane monohydrate in a dose of 150 mg/kg. Melatonin was administered intragastrally at a dose of 10 mg/kg body weight for 7 and 14 days. In blood erythrocyte hemolysis, the content of reduced glutathione (GSH), glutathione-Stransferase (GST), glutathione peroxidase (GPX), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G-6-PD) and glucose-6-phosphate dehydrogenase (G-6-PD) activity were determined and plasma activity GST, the content of SH-groups and hydrogen sulfide (H2S).Results and discussion. In blood of rats at alloxane induced diabetes, an increase in the activity of enzymes GST, GPX, GR and G-6-PD in red blood cells was observed when the concentration of GSH decreased. Along with this, there was a decrease in the levels of SH-groups and H2S and GST activity in the blood plasma of the alloxan-diabetic group. The results showed that both on day 7 and day 14 of the experiment, melatonin normalizes the glutathione system of blood in ratswith alloxane-induced diabetes. Also, the administration of melatonin for 7 days to the alloxane-diabetic group of rats normalized the levels of H2S and SH-groups of plasma. On the 14th day of the experiment there was an increase in the level of these two indicators, but they significantly differed from those of the control group.Conclusions. Introduction of melatonin to the alloxanediabetic group helps to normalize the studied parameters, possibly due to its antioxidant properties and the ability to protect against oxidative stress, which has a positive effect on the glutathione system and the level of SH-groups and H2S.