Some Effects of Heat and Ionizing Radiation on Spores of Bacillus Megaterium

Spores of B. megaterium, irradiated at dose levels sufficient to destroy the capacity for cell division, may retain the ability to germinate in glucose. When suspensions of such irradiated spores germinate, they consume oxygen at a rate approximately equal to that of unirradiated spores, stain, lose dipicolinic acid, and decrease in optical density. Higher levels of irradiation may alter spore permeability, simulating the responses characterizing germination even in the absence of glucose. The enhancement of radiation effects by subsequent heating and similarities in the effects of heat and radiation are noted. (auth)