The feasibility of trace metal speciation in proteins directly using flow field-flow fractionation-inductively coupled plasma mass spectrometry (FlFFF-ICP-MS) was investigated. The effect on the separation of the FlFFF system cross flow rate was demonstrated for a carbonic anhydrase and alcohol dehydrogenase mixture. Preliminary studies with FlFFF-ICP-MS were applied to several protein standards including metallothionein, carbonic anhydrase, ceruloplasmin, alcohol dehydrogenase, and thyroglobulin. Cadmium was detected in metallothionein; copper and zinc in carbonic anhydrase, alcohol dehydrogenase, and ceruloplasmin, and iodide in thyroglobulin.
No takes yet. Share an insight, caveat, or question.
Siripinyanond et al. (1999) studied this question.
Synapse has enriched 5 related papers. Consider them for comparative context: