Answers are grounded in this paper and cite their sources.
A method for the separation and determination of biliary metabolites of equilin in rat by high-performance liquid chro-matography with electrochemical detection has been developed. After extraction of equine estrogens in bile with a C18 bonded silica cartridge, conjugated metabolites were hydrolyz-ed with sulfatase containing β-glucuronidase and free estrogens liberated were then separated on a reversed phase C18 column, the limit of detection being in the range of 5–20 pg. The transformation of equilin administered orally into 2-methoxy derivatives of equilin, equilenin and their 17β-reduced metabolites is also discussed.
No takes yet. Share an insight, caveat, or question.
Ikegawa et al. (1994) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: