What question did this study set out to answer?

To evaluate the in vitro antimalarial activity and synergistic efficacy of pyrimethamine and sulfadoxine against sensitive and resistant strains of Plasmodium falciparum under varying folic acid and PABA conditions.

May 1, 1984American Journal of Tropical Medicine and Hygiene

Synergistic Antimalarial Activity of Pyrimethamine and Sulfadoxine against Plasmodium falciparum In Vitro

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Authors

JCJeffrey D. ChulayUniversity of Maryland, Baltimore WWWilliam M. WatkinsUniversity of Nairobi DSDavid SixsmithKenya Medical Research Institute

Discussion

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Implication

In vitro experimental study demonstrates synergistic inhibition by sulfadoxine and pyrimethamine against Plasmodium falciparum, indicating parasite utilization of exogenous folic acid.

Key Points

To evaluate the in vitro antimalarial activity and synergistic efficacy of pyrimethamine and sulfadoxine against sensitive and resistant strains of Plasmodium falciparum under varying folic acid and PABA conditions.
Tested pyrimethamine-sensitive and pyrimethamine-resistant strains of Plasmodium falciparum in vitro.
Assessed parasite growth inhibition across varying concentrations of pyrimethamine, sulfadoxine, and combinations in media with or without p-aminobenzoic acid (PABA) and folic acid.
Parasites grown in medium devoid of folic acid and PABA were inhibited by 10⁻⁶ M sulfadoxine.
Addition of physiological folic acid (0.01 mg/L) reduced sulfadoxine activity 1,000-fold, whereas a 100-fold higher folic acid concentration reduced pyrimethamine activity 10-fold.
Sulfadoxine at 10⁻⁷ M potentiated pyrimethamine activity in PABA-free medium both in the absence of folic acid and in the presence of 0.01 mg/L folic acid.

Cite This Study

Chulay et al. (1984) studied this question.

synapsesocial.com/papers/6a72853ffebe604dd70aa61c https://doi.org/10.4269/ajtmh.1984.33.325

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May 1, 1984American Journal of Tropical Medicine and Hygiene

Synergistic Antimalarial Activity of Pyrimethamine and Sulfadoxine against Plasmodium falciparum In Vitro

View Full Paper

Ask AI

Bookmark

Authors

JCJeffrey D. ChulayUniversity of Maryland, Baltimore WWWilliam M. WatkinsUniversity of Nairobi DSDavid SixsmithKenya Medical Research Institute

Discussion

Member takes

Implication

In vitro experimental study demonstrates synergistic inhibition by sulfadoxine and pyrimethamine against Plasmodium falciparum, indicating parasite utilization of exogenous folic acid.

Key Points

To evaluate the in vitro antimalarial activity and synergistic efficacy of pyrimethamine and sulfadoxine against sensitive and resistant strains of Plasmodium falciparum under varying folic acid and PABA conditions.
Tested pyrimethamine-sensitive and pyrimethamine-resistant strains of Plasmodium falciparum in vitro.
Assessed parasite growth inhibition across varying concentrations of pyrimethamine, sulfadoxine, and combinations in media with or without p-aminobenzoic acid (PABA) and folic acid.
Parasites grown in medium devoid of folic acid and PABA were inhibited by 10⁻⁶ M sulfadoxine.
Addition of physiological folic acid (0.01 mg/L) reduced sulfadoxine activity 1,000-fold, whereas a 100-fold higher folic acid concentration reduced pyrimethamine activity 10-fold.
Sulfadoxine at 10⁻⁷ M potentiated pyrimethamine activity in PABA-free medium both in the absence of folic acid and in the presence of 0.01 mg/L folic acid.

Cite This Study

Chulay et al. (1984) studied this question.

synapsesocial.com/papers/6a72853ffebe604dd70aa61c https://doi.org/10.4269/ajtmh.1984.33.325

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