January 1, 1991Nucleic Acids ResearchOpen Access

Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin

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Population

Dystrophin cDNA in a pEX2 plasmid (bacterial transformants expressing truncated dystrophin fusion proteins)

Design

Preclinical

Authors

Discussion

Hypothesis-generating in animal models; leaves open whether mapped dystrophin epitopes inform human antibody therapies.

Population

Dystrophin cDNA in a pEX2 plasmid (bacterial transformants expressing truncated dystrophin fusion proteins)

Intervention

Transposon mutagenesis using Tn1000 transposons carrying translation termination codons

Outcome

Mapping of antibody-binding epitopes on the dystrophin protein

Transposon mutagenesis provides a rapid method for mapping epitopes on large proteins like dystrophin without in vitro DNA manipulation.

Sedgwick et al. (1991) studied this question.

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