Novel polymerase spiral reaction assay for the visible molecular detection of porcine circovirus type 3

Fig. 1. Sensitivity of the polymerase spiral reaction (PSR) and loop mediated isothermal amplification for the detection of Porcine circovirus type 3 (PCV3). Ten-fold serial dilutions of standard plasmid (PCV3-ORF2) were subjected to the PSR and LAMP assays and analyzed. Lane M, molecular size marker DL2000. Lanes 1–7, dilutions of PCV3-ORF2 (1.13 × 107 copies, 1.13 × 106 copies, 1.13 × 105 copies, 1.13 × 104 copies, 1.13 × 103 copies, 1.13 × 102 copies, and 1.13 × 101 copies); and lane N, negative control. a Agarose gel electrophoresis demonstrating the sensitivity of the PSR assay. b Colorimetric analysis demonstrating the sensitivity of the PSR assay. c Agarose gel electrophoresis demonstrating the sensitivity of the LAMP assay

Fig. 2. Determination of PSR specificity. Lane M, molecular size marker DL2000; lane 1, PCV3; lane 2, pseudorabies virus (PRV); lane 3, porcine reproductive and respiratory syndrome virus (PRRSV); lane 4, porcine circovirus type 2 (PCV2); lane 5, classical swine fever virus (CSFV); lane 6, porcine epidemic diarrhea virus (PEDV); and lane N, negative control. a Specificity of the PSR assay as determined by electrophoretic separation of the reaction products. b Colorimetric analysis of the reactions demonstrating the specificity of the PSR assay