What question did this study set out to answer?

To identify downstream effector and signaling pathways linked to inflammatory resolution following CD163-mediated hemoglobin clearance in human monocytes and macrophages.

December 2, 2003Circulation Research

Hemoglobin Scavenger Receptor CD163 Mediates Interleukin-10 Release and Heme Oxygenase-1 Synthesis

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Authors

PPPandelis PhilippidisImperial College London JMJustin C. MasonImperial College London BEBetsy EvansLeeds General Infirmary

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Implication

Translational study reveals CD163-mediated interleukin-10 release and heme oxygenase-1 induction in human macrophages, indicating a novel anti-inflammatory pathway during inflammation resolution.

Key Points

To identify downstream effector and signaling pathways linked to inflammatory resolution following CD163-mediated hemoglobin clearance in human monocytes and macrophages.
Assessed CD163 expression across in vitro cultured human monocyte-derived macrophages, in vivo resolving cantharidin-induced skin blisters, and circulating monocytes from patients undergoing cardiopulmonary bypass surgery.
Stimulated CD163-expressing cells with hemoglobin:haptoglobin complexes with or without the neutralizing anti-CD163 antibody RM3/1 to measure interleukin-10 release and heme oxygenase-1 expression.
Monocyte differentiation into macrophages in vitro and within resolving skin blisters increased CD163 expression by more than 15-fold.
Hemoglobin:haptoglobin binding to CD163 elicited strong interleukin-10 secretion—completely blocked by anti-CD163 antibodies—which subsequently triggered heme oxygenase-1 synthesis via an autocrine mechanism.
Heme oxygenase-1 induction occurred in vivo 24 to 48 hours after cardiopulmonary bypass onset, matching the peak resolution phase of systemic inflammation.

Cite This Study

Philippidis et al. (2003) studied this question.

synapsesocial.com/papers/6a728f8775498292b70b99ce https://doi.org/10.1161/01.res.0000109414.78907.f9

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Implication

Translational study reveals CD163-mediated interleukin-10 release and heme oxygenase-1 induction in human macrophages, indicating a novel anti-inflammatory pathway during inflammation resolution.

Hemoglobin Scavenger Receptor CD163 Mediates Interleukin-10 Release and Heme Oxygenase-1 Synthesis

Discussion

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Implication

Key Points

Cite This Study

Hemoglobin Scavenger Receptor CD163 Mediates Interleukin-10 Release and Heme Oxygenase-1 Synthesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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