April 1, 1983The Journal of Cell BiologyOpen Access

Effects of EDTA treatment upon the protein subunit composition and mechanical properties of mammalian single skeletal muscle fibers

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Population

Skinned single skeletal muscle fibers from rabbit psoas muscles

Comparison

EDTA treatment followed by readdition of… vs Baseline measurements prior to LC(2) removal

Design

Preclinical

Authors

RMR L MossUniversity of Wisconsin–Madison GGG G GiulianUniversity of Wisconsin–Madison MGML Greaser

Discussion

Member takes

Implication

Supports LC(2) role in skeletal muscle kinetics; leaves open translation to human cardiac contractility.

Structured PICO

Population

Skinned single skeletal muscle fibers from rabbit psoas muscles

Intervention

EDTA treatment (20 mM EDTA buffer solution at 30 degrees C for up to 240 min) followed by readdition of Troponin C (TnC) and myosin light chain LC(2)

Comparator

Baseline measurements prior to LC(2) removal

Outcome

Isometric tension and maximum velocity of shortening (V(max))surrogate

Removal of myosin light chain LC(2) from rabbit skeletal muscle fibers reduces maximum shortening velocity, indicating its role in myosin-actin interaction kinetics.

Cite This Study

Moss et al. (1983) studied this question.

synapsesocial.com/papers/6a729add2163a0a01bc6323b https://doi.org/10.1083/jcb.96.4.970

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Effects of EDTA treatment upon the protein subunit composition and mechanical properties of mammalian single skeletal muscle fibers

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Effects of EDTA treatment upon the protein subunit composition and mechanical properties of mammalian single skeletal muscle fibers

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider