What question did this study set out to answer?

To identify and characterize the genes encoding lateral flagella in Aeromonas species and determine their role in epithelial cell adherence and biofilm formation.

January 1, 2002Molecular MicrobiologyOpen Access

Lateral flagella of Aeromonas species are essential for epithelial cell adherence and biofilm formation

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Authors

RGRosalina Gavı́nBiomedical Research Networking Center on Neurodegenerative Diseases ARAli A. RabaanAlfaisal University SMSusana MerinoUniversitat de Barcelona

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Implication

Mutational analysis demonstrates that lateral flagella mediate adherence and biofilm formation in Aeromonas species, indicating their structural and functional dependence on polar flagella.

Key Points

To identify and characterize the genes encoding lateral flagella in Aeromonas species and determine their role in epithelial cell adherence and biofilm formation.
Isolated and sequenced lateral flagellar gene clusters from Aeromonas hydrophila (lafA-U) and Aeromonas caviae (lafA1, lafA2, lafB, and fliU).
Generated targeted gene and transposon insertion mutants to evaluate flagellar morphology, bacterial motility, epithelial adherence, and biofilm formation.
Disruption of lafB, lafS, or both lateral flagellins eliminated lateral flagella expression and led to a marked reduction in adherence and biofilm formation.
Mutations in lafA1, lafA2, fliU, or lafT preserved lateral flagella production but still significantly impaired bacterial adherence levels.
Mutations that prevented polar flagellum synthesis completely abolished lateral flagella expression, demonstrating shared motor proteins and coordinated regulation.

Cite This Study

Gavı́n et al. (2002) studied this question.

synapsesocial.com/papers/6a72a3b7e36a167817e3fcbb https://doi.org/10.1046/j.1365-2958.2002.02750.x

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Implication

Mutational analysis demonstrates that lateral flagella mediate adherence and biofilm formation in Aeromonas species, indicating their structural and functional dependence on polar flagella.

Lateral flagella of Aeromonas species are essential for epithelial cell adherence and biofilm formation

Discussion

Member takes

Implication

Key Points

Cite This Study

Lateral flagella of Aeromonas species are essential for epithelial cell adherence and biofilm formation

Discussion

Member takes

Implication

Key Points

Cite This Study

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