Disposable non-enzymatic electrochemical glucose sensors based on screen-printed graphite macroelectrodes modified via a facile methodology with Ni, Cu, and Ni/Cu hydroxides are shown to accurately determine glucose in real human serum blood samples

and the lowest detection limit of 0.2 μM (S/N = 3). Excellent selectivity was evident against common interfering species, and it was shown to be possible to obtain satisfactory results in human blood serum samples using the as-fabricated sensors. The low cost of the SPEs, the facile preparation and observed clinically relevant analytical sensitivities and limit of detections towards the sensing of glucose make these screen-printed macroelectrode based electrochemical sensing platforms promising for routine human blood serum glucose analysis.